Coding

Part:BBa_K2000005:Design

Designed by: Qin YE   Group: iGEM16_JSNU-China   (2016-10-10)


hKLF4-FL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1464
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 313


Design Notes

to epress whole KLF4 gene and get correct protein.


Source

at gaggcagcca

     61 cctggcgagt ctgacatggc tgtcagcgac gcgctgctcc cgtccttctc cacgttcgcg

    121 tccggcccgg cgggaaggga gaagacactg cgtccagcag gtgccccgaa taaccgctgg

    181 cgggaggagc tctcccacat gaagcgactt cccccggtgc ttcccggccg cccctacgac

    241 ctggcggcgg cgaccgtggc caccgacctg gagagtggag gagtcggcgc ggcttgcggc

    301 agcagcaacc cggctcttct gccccggagg gagacggagg agttcaatga tctcctggac

    361 ctggacttta tcctctccaa ctcgctgtcc catcaggagt ccgtggccgc cacggtgtcc

    421 tcctcggcat cagcctcatc ctcgtcctcc ccgtcgagca gcggtccagc cagtgcgccc

    481 tccacctgcc gc ttcagcta tccaatccgg gccgggggcg acccgggcgt ggcggcgccg

    541 ggcggcgcgg gcggcggcct cctgtatggc cgggagtctg caccccctcc gacagctccc

    601 ttcaacctgg cggacatcaa cgatgtgagc ccctccggcg gcttcgtggc cgagctcctg

    661 cggcccgaat tggacccagt gtacattccg ccgcagcagc cgcagccgcc aggtggcggg

    721 ctgatgggca agttcgtgtt gaaggcgtcg ctgagtgccc ctggcagcga gtacggcagc

    781 ccgtcggtca tcagtgttag caaaggcagc ccagacggca gccacccggt ggtggtggcg

    841 ccctacagcg gcgggccgcc acgcatgtgc ccgaagatca agcaggagac tgtctcctcg

    901 tgcaccgtcg gtcggcccct agaggcccac ttgggcactg gacctcctct cagcaatggc

    961 caccggccac ctgcgcacga ctttcccttg gggcggcagc tccccagcag gactaccccg

   1021 accctgggtg ccgaggaact gctgagcagc cgggactgtc atcctgccct gccgctcccc

   1081 ccgggcttcc atccccaccc cgggcccaac taccctccct tcctgcccga ccagatgcag

   1141 ccgcaggtcc caccgctgca ttaccaagag ctcatgccac ccggttcctg catgccggag

   1201 gagcccaaac caaagagggg aaggcggtcg tggccccgga aaaggacggc cactcacact

   1261 tgtgattatg caggctgcgg caaaacctac acgaagagtt ctcatctcaa ggcacacctg

   1321 cgcacccaca caggtgagaa accttaccac tgtgactggg atggctgtgg gtggaagttt

   1381 gcccgctcag atgaactgac caggcactat cgcaaacaca ctgggcaccg ccccttccag

   1441 tgccagaaat gcgaccgggc attctcgcgg tcggaccacc tcgccttaca tatgaaaagg

   1501 cacttttaaa

References